بررسی تأثیر جانشین پوستی تهیه شده از کشت هم‌زمان کراتینوسیت و فیبروبلاست روی داربست کلاژنی در ترمیم زخم پوست موش صحرایی

Abstract Background: Considering the ineffective conventional therapeutic methods for treating different types of wounds, and because of reports of successful use of skin substitutes prepared from co-cultured keratinocytes and fibroblasts on the scaffold of collagen, this study was performed to evaluate the skin substitute on experimentally induced wounds in rats. Materials and methods: This experimental study was performed on five albino Wistar rats with a 2 × 2 cm2 dimension wounds on their backs. In the experimental group skin substitutes, prepared from co-cultured fibroblasts and keratinocytes on a collagen scaffold, were used no intervention was done in the control group. Wound healing was assessed histopathologically on 10th day with qualitative indicators, like vascularization, fibrosis, and necrosis, and quantitative indicators, like epithelial thickness, number of hair follicles, fibroblasts, keratinocytes, lymphocytes and neutrophils. Descriptive statistics, Mann-Whitney U Test, and t-test were used to analyze the data. Results: In all parameters studied, wound healing in the experimental group was better than the control group. Fibrosis severity of the experimental group was less than control group (p> 0.05), thickness of the epithelial layer in the control group (30.4 ± 20.4) was less than experimental group (180.5 ± 73.8) (p> 0.01) and also the number of fibroblasts and keratinocytes in the experimental group was more than controls (p> 0.05). Conclusion: It seems that skin substitutes, prepared from co-cultured fibroblasts and keratinocytes on a collagen scaffold, can improve and accelerate full-thickness wound closure. We recommend further studies in this field. Keywords: Fibroblast, Keratinocyte, Collagen-scaffold, Wound healing.